functional expression of delayed rectifier potassium channels in cardiomyocytes ‎derivedfromembryonicstemcells

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Kv2 channels form delayed-rectifier potassium channels in situ.

A non inactivating potassium current known as the delayed rectifier plays a major role in membrane repolarization during an action potential. Whereas several candidate genes exist that code for potassium current, the identities of the molecular isotypes that are responsible in situ for membrane repolarization remain unidentified. We report that Kv2 channels play a major role in action potential...

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functional expression of potassium channels in cardiomyocytes derived from embryonic stem cells

royan b 1 stem cell can be differentiated to specialized cell types including cardiomyocytes. this developmental change is accompanied with expression of various k + channel types. the aim of this study was to detect functional expression of k + currents from stem cell stage and one week and two weeks after differentiation into cardiomyocyte. mouse stem cell derived cardiomyocytes (es-cardiomyo...

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Inward rectifier potassium channels. Cloning, expression and structure-function studies.

A PCR-based cloning strategy was used to identify novel subunits of the two-transmembrane domain inward rectifier potassium channel family from rat brain, heart, and skeletal muscle. When expressed in Xenopus oocytes, two of these clones (Kir4.1 and Kir2.3) gave rise to inwardly rectifying potassium currents. Two-electrode voltage clamp commands to potentials negative to EK evoked inward potass...

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Functional expression of potassium channels in cardiomyocytes derived from embryonic stem cells

Royan B(1) stem cell can be differentiated to specialized cell types including cardiomyocytes. This developmental change is accompanied with expression of various K(+) channel types. The aim of this study was to detect functional expression of K(+) currents from stem cell stage and one week and two weeks after differentiation into cardiomyocyte. Mouse stem cell derived cardiomyocytes (ES-cardio...

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Functional characterization of inward rectifier potassium ion channel in murine fetal ventricular cardiomyocytes.

AIMS Previous studies have shown the dramatic changes in electrical properties of murine fetal cardiomyocytes, while details on inward rectifier potassium current (IK1) are still seldom discussed. Thus we aimed to characterize the functional expression and functional role of IK1 in murine fetal ventricular cardiomyocytes. METHODS Whole cell patch clamp was applied to investigate the electroph...

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Effects of Pb2+ on delayed-rectifier potassium channels in acutely isolated hippocampal neurons.

Effects of Pb2+ on delayed-rectifier potassium channels in acutely isolated hippocampal neurons. J. Neurophysiol. 78: 2649-2654, 1997. The effects of Pb2+ on delayed-rectifier potassium currents were studied in acutely isolated hippocampal neurons (CA1 neurons, CA3 neurons, granule cells) from the guinea pig using the patch-clamp technique in the whole cell configuration. Pb2+ in micromolar con...

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عنوان ژورنال:
research in pharmaceutical sciences

جلد ۷، شماره ۵، صفحات ۰-۰

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